Multiplexed Mutagenically Separated PCR: Simultaneous Single-Tube Detection of the Factor V R506Q (G1691A), the Prothrombin G20210A, and the Methylenetetrahydrofolate Reductase A223V (C677T) Variants
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چکیده
منابع مشابه
Acs:180 Results Obtained for Macroprolactinemic Samples Multiplexed Mutagenically Separated Pcr: Simulta- Neous Single-tube Detection of the Factor V R506q (g1691a), the Prothrombin G20210a, and the Methyl- Enetetrahydrofolate Reductase A223v (c677t) Variants
(range, 250% to 86%) indicates a highly variable, sampledependent response of the ACS:180 assay to macroprolactin. The great disparity of values observed when comparing results from macroprolactinemic samples measured by the Elecsys or the Immulite assay with the results obtained by a low-reading method such as ACS:180 may reflect variation in the structure of macroprolactin. Macroprolactin is ...
متن کاملSimultaneous determination of methylenetetrahydrofolate reductase C677T and factor V G1691A genotypes by mutagenically separated PCR and multiple-injection capillary electrophoresis.
The C677T mutation of the methylenetetrahydrofolate reductase gene and the G1691A (Leiden) mutation of the factor V gene are established risk factors for thromboembolic disease. We here present an assay for the simultaneous genotyping of these common genetic variants. The assay involves a strategy based on multiplex mutagenically separated PCR performed in a single tube containing six primers. ...
متن کاملRelationship between Methylenetetrahydrofolate Reductase (C677T), Factor V Leiden (G1691A), Prothrombin Mutation (G20210A) and Severe Preeclampsia in a Brazilian Population
Objectives: To better understand the etiologic factors that can influence preeclampsia, we investigated hereditary factors for thrombosis, FV Leiden, F II 20210A mutation and the polymorphism C677T of the MTHFR, as singly and as in association, in a group of women from Ceará stateNortheast Brazil with severe preeclampsia. Material and Methods: We conducted a case-control study. 101 cases of sev...
متن کاملRapid detection of prothrombotic mutations of prothrombin (G20210A), factor V (G1691A), and methylenetetrahydrofolate reductase (C677T) by real-time fluorescence PCR with the LightCycler.
storage of blood at room temperature is uncertain, but one possibility is that cleavage of the antigen occurs, releasing smaller fragments, each with multiple OVX1 epitopes. Different cutoffs at 7.2, 10.5, or 12.1 kilounits/L have been used in clinical studies (2, 4). The present findings, therefore, are clinically relevant because the assay range is narrow and the effect we have noted can caus...
متن کاملFrequency of factor V Leiden (G1691A) and prothrombin (G20210A) polymorphisms in Population of Kerman Province, Iran
Background & Aims:Thromboembolism is an acute cardiovascular disease that ranges from clinically unimportant to massive embolism. Both acquired and hereditary risk factors contribute to the disease. We aimed to determine the prevalence of two hereditary predisposing factor of the disease, prothrombin G20210A and factor V Leiden (G1691A) polymorphisms, in Kerman population.<br /...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2001
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/47.2.333